Composite

Part:BBa_K3032072

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-15)


CarH repressor under medium anderson (0.33) promoter (Ready for Expression)

This is a ready to use composite part of CarH repressor under medium Anderson promoter (BBa_J23110) and strong RBS (BBa_B0034). Cobalamin must present in medium for CarH oligomerization.

CarH has cobalamine (Vitamin B12), which has a photosensitive Co-C bond, as a prosthetic group. In the dark, CarH is a tetramer that binds strongly to DNA and represses protein translation. When excited by green light, the photosensitive Co-C bond breaks, this disrupts the tetramer structure, and then gene expression can start.

CarH repression tested with Bba_K3032070.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None